Cat#:RP-3761H;Product Name:Recombinant Human HGF Protein, CHO;Synonym:Scatter Factor (SF), Hepatopoietin (HPTA), HGF.;Description:Hepatocyte Growth Factor Protein produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of approximately 75kDa. The HGF is purified by proprietary chromatographic techniques.?;Source:Chinese Hamster Ovarian Cells.;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.;Formulation:The protein was lyophilized from a concentrated (292µg/ml) solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;