Cat#:RP-3762H;Product Name:Recombinant Human HGF Protein, HEK;Synonym:Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF.;Description:HGF Protein produced in HEK cells is a glycosylated peptide, containing 697 a.a. (Gln-32 to Ser-728) having a total molecular weight of 70kDa. The HGF is purified by proprietary chromatographic techniques.;Source:HEK.;AA Sequence:32-QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR;Purity:Greater than 95% as obsereved by SDS-PAGE.;Bioactivity:The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 and is typically 10-50ng/ml.;Formulation:The HGF was lyophilized from 1mg/ml in 1xPBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized HGF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.;Storage:Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
HGF Protein produced in HEK cells is a glycosylated peptide, containing 697 a.a. (Gln-32 to Ser-728) having a total molecular weight of 70kDa. The HGF is purified by proprietary chromatographic techniques.
The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 and is typically 10-50ng/ml.
Formulation:
The HGF was lyophilized from 1mg/ml in 1xPBS.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized HGF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Storage:
Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.