Cat#:RP-6204H;Product Name:Recombinant Human TPO Protein, HEK;Synonym:Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.;Description:Thrombopoietin Protein produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation. The TPO is purified by proprietary chromatographic techniques.;Source:HEK.;Purity:Greater than 95% as obsereved by SDS-PAGE.;Bioactivity:The activity was determined by the dose-dependent stimulation of the proliferation of MO7e cells, the EC50 is 3.8ng/ml.;Formulation:The TPO protein was filtered (0.2µm) and lyophilized from 0.73mg/ml in 1xPBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Thrombopoietin in sterile PBS containing 0.1% endotoxin-free recombinant HSA.;Storage:Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Thrombopoietin Protein produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation. The TPO is purified by proprietary chromatographic techniques.
Source:
HEK.
Purity:
Greater than 95% as obsereved by SDS-PAGE.
Bioactivity:
The activity was determined by the dose-dependent stimulation of the proliferation of MO7e cells, the EC50 is 3.8ng/ml.
Formulation:
The TPO protein was filtered (0.2µm) and lyophilized from 0.73mg/ml in 1xPBS.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized Thrombopoietin in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Storage:
Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.