Cat#:RP-5108H;Product Name:Recombinant Human PLGF2 Protein, CHO;Synonym:PIGF, PGF, PlGF-2, PLGF-2.;Description:PLGF2 Protein (19-170 a.a.) produced in CHO is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 33kDa. The PLGF-2 Protein protein is purified by proprietary chromatographic techniques.;Source:Chinese Hamster Ovary cells (CHO).;AA Sequence:LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR.;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:The ED50, as measured in a cell proliferation assay using MDA?MB?231 cells is less than 10µg/ml.;Formulation:PLGF-2 protein was lyophilized from a 0.2µm filtered solution in HCl.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized PLGF2 at 200µg/ml in PBS, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized PLGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
PLGF2 Protein (19-170 a.a.) produced in CHO is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 33kDa. The PLGF-2 Protein protein is purified by proprietary chromatographic techniques.
The ED50, as measured in a cell proliferation assay using MDA?MB?231 cells is less than 10µg/ml.
Formulation:
PLGF-2 protein was lyophilized from a 0.2µm filtered solution in HCl.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized PLGF2 at 200µg/ml in PBS, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized PLGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.