Cat#:RPH-NP324;Product Name:Recombinant Human Pre-B-Cell Colony-Enhancing Factor 1 / PBEF / Visfatin Protein;Synonym:Pre-B cell-enhancing factor, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, NAMPT, PBEF, PBEF1;Description:Recombinant Human Visfatin Protein is produced in E.coli and the target gene encoding Met1-His491 is expressed with a His tag at the N-terminus.;Source:E.coli;AA Sequence:MNHKVHHHHHHMNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKY EETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAV PEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLD GLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHST ITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDS GNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIA FGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLE EGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH;Purity:Greater than 95% as determined by reducing SDS-PAGE.;Endotoxin:< 0.1 ng/µg (1 IEU/µg) as determined by LAL test.;Formulation:Recombinant Human Pre-B-Cell Colony-Enhancing Factor 1/PBEF/Visfatin Protein was lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH8.0.;Stability:Recombinant Human Pre-B-Cell Colony-Enhancing Factor 1/PBEF/Visfatin Protein is stable for up to 1 year from date of receipt at -70℃.;Reconstitution:Please centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.;Storage:Lyophilized recombinant human PBEF protein should be stored below -20°C, though stable at room temperature for 3 weeks. Reconstituted recombinant human PBEF protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted recombinant human Visfatin protein samples are stable below -20°C for 3 months.;Usage:For Lab Research Use Only;
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:
< 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Formulation:
Recombinant Human Pre-B-Cell Colony-Enhancing Factor 1/PBEF/Visfatin Protein was lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH8.0.
Stability:
Recombinant Human Pre-B-Cell Colony-Enhancing Factor 1/PBEF/Visfatin Protein is stable for up to 1 year from date of receipt at -70℃.
Reconstitution:
Please centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Usage:
For Lab Research Use Only
Storage:
Lyophilized recombinant human PBEF protein should be stored below -20°C, though stable at room temperature for 3 weeks. Reconstituted recombinant human PBEF protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted recombinant human Visfatin protein samples are stable below -20°C for 3 months.