Cat#:RP-1825H;Product Name:Recombinant Human IGF1 Protein;Synonym:Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.;Description:Recombinant human Insulin-Like Growth Factor-1 / IGF1 Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kDa. Recombinant human IGF1 protein is purified by our unique chromatographic techniques.;Source:E.coli ;AA Sequence:GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.;Purity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE.;Bioactivity:The ED50 of recombinant human IGF1 protein as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1×106 units/mg.;Formulation:The recombinant human IGF1 protein was lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.0, 130mM NaCl.;Stability:Recombinant Human IGF1 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human Insulin-Like Growth Factor-1/IGF1 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human IGF1 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;References:Yakar S, Rosen CJ, Beamer WG, Ackert-Bicknell CL, Wu Y, Liu JL, Ooi GT, Setser J, Frystyk J, Boisclair YR, LeRoith D (2002). "Circulating levels of IGF-1 directly regulate bone growth and density". Journal of Clinical Investigation. 110 (6): 771–781.;