Cat#:RP-3991H;Product Name:Recombinant Human IGF1 Protein Des1-3;Synonym:Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF, Des(1-3), Des1-3, Des 1-3, Des (1-3), IGF-1 (4-70).;Description:Recombinant human IGF-I Des(1-3) Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids (aa 4-70) and having a molecular mass of 7368.5 Dalton. Recombinant human IGF-1 Des1-3 is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA.;Purity:Greater than 97.0% as determined by RP-HPLC and SDS-PAGE.;Bioactivity:The ED50 of recombinant human IGF1 protein as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5×105 units/mg.;Formulation:The recombinant human IGF1 protein (1mg/ml) was lyophilized was lyophilized from a 0.2µm filtered concentrated solution in 20mM PBS, pH 7.0.;Stability:Recombinant human IGF1 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the recombinant human IGF-I des(1-3) in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human IGF-I des(1-3) protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human IGF1 des-1-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;References:Yakar S, Rosen CJ, Beamer WG, Ackert-Bicknell CL, Wu Y, Liu JL, Ooi GT, Setser J, Frystyk J, Boisclair YR, LeRoith D (2002). "Circulating levels of IGF-1 directly regulate bone growth and density". Journal of Clinical Investigation. 110 (6): 771–781.;