Cat#:RP-3313H;Product Name:Recombinant Human G CSF Protein, CHO;Synonym:CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.;Description:Granulocyte Colony Stimulating Factor Protein produced in CHO cells?is a single, glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of approximately 18 kDa. G-CSF is purified by proprietary chromatographic techniques.;Source:Chinese Hamster Ovary Cells (CHO).;AA Sequence:TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP.;Purity:Greater than 97.0% as determined by: a) Analysis by RP-HPLC. b) Analysis by SDS-PAGE.;Bioactivity:The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg.;Formulation:G-CSF was lyophilized from a concentrated (1mg/ml) Phosphate- Buffered Saline, pH 7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution G-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;