Cat#:RP-2171H;Product Name:Recombinant Human BAFF Protein, Plant;Synonym:BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, B-cell Activating Factor.;Description:BAFF Protein produced in Nicotiana benthamiana plant is a single glycosilated polypeptide chain containing 151 amino acids fragment?(134-285). BAFF (C830H1277N223O242S5) is fused to a 10-His-tag at the N-terminal having the total molecular mass of 18-20kDa and purified by standard chromatographic techniques.;Source:Nicotiana benthamiana plant;AA Sequence:HHHHHHHHHH AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL;Purity:Greater than 97.0% as determined by Analysis by SDS-PAGE.;Bioactivity:The activity is determined by dose-dependent stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. ED50 ? 50ng/ml;Formulation:Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7 and 0.2 M NaCl.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized BAFF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized?BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution?BAFF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;