Indirect ELISA Kit

The Indirect ELISA Kit is a widely used assay system designed for the detection and quantification of specific analytes such as proteins, antibodies, and other biomolecules within complex biological samples. This kit utilizes a two-step detection process that enhances sensitivity and flexibility compared to the direct ELISA method.

In an indirect ELISA, the process begins with the immobilization of the target antigen on the surface of the plate. A primary antibody, specific to the antigen, is then added to bind the target. After a washing step to remove unbound antibodies, a labeled secondary antibody is introduced. This secondary antibody is designed to recognize and bind to the host species of the primary antibody. The secondary antibody is typically conjugated to an enzyme, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Following a second wash step to remove unbound secondary antibodies, a substrate is added. The enzyme acts on the substrate to produce a color change, which can be measured to determine the presence and quantity of the target analyte.

Advantages of Indirect ELISA:

Higher Sensitivity: The use of a secondary antibody amplifies the signal, making indirect ELISA more sensitive than direct ELISA, allowing for the detection of lower concentrations of the target analyte.
Cost-Effective: Since the secondary antibodies are often commercially available and can be used with a variety of primary antibodies, this method is generally less expensive than direct ELISA.
Flexibility: The method is versatile, as it allows the use of many different primary antibodies, making it suitable for a wide range of applications.

Disadvantages of Indirect ELISA:

Risk of Cross-Reactivity: The primary disadvantage is the potential for cross-reactivity between the secondary antibody and other proteins in the sample, which can lead to nonspecific signals and reduced accuracy.

The Indirect ELISA Kit is particularly useful for applications where high sensitivity is required and flexibility in antibody selection is advantageous.

CAT. #
PRODUCT NAME
SAMPIE
PRICE

EIA-218C
Poliomyelitis; Poliomyelitis vieus; Polio
serum and plasma
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EIA-244C
Mycobacterium tuberculosis IgM ELISA Kit, Sensitive
serum and plasma
+Inquiry

EIA-245C
Mycobacterium tuberculosis IgM ELISA Kit
serum and plasma
+Inquiry

EIA-246C
Mycobacterium tuberculosis IgG ELISA Kit, Sensitive
serum and plasma
+Inquiry

EIA-247C
Mycobacterium tuberculosis IgG ELISA Kit
serum and plasma
+Inquiry

EIA-248C
Mycobacterium tuberculosis IgA ELISA Kit, Sensitive
serum and plasma
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EIA-249C
Mycobacterium tuberculosis IgA ELISA Kit
serum and plasma
+Inquiry

EIA-253C
MVV / CAEV ELISA Kit
sheep serum and goat serum
+Inquiry

EIA-566C
Human CMV IgG(CSF) ELISA kit
serum and cerebrospinal fluid
+Inquiry

EIA-569C
Human CMV IgA ELISA kit
serum and cerobrospinal fluid
+Inquiry

EIA-590C
Human Borrelia ELISA Kit
PLASMA, SERUM OR CEREBROSPINAL FLUID
+Inquiry

EIA-598C
Human B. Burgdorferi ELISA Kit
PLASMA, SERUM OR CEREBROSPINAL FLUID
+Inquiry

EIA-350C
MC-LR ELISA Kit
environmental samples
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EIA-351C
MAP ELISA kit
erum, plasma and milk samples from cattle, sheep and goat
+Inquiry

EIA-638C
HiB IgG ELISA Kit
Human Serum, Citrate-, EDTA- or Heparin Plasma
+Inquiry

EIA-385C
Human VCA EBV (CSF) IgG ELISA kit
human serum and cerebrospinal fluid
+Inquiry

EIA-656C
HCV ELISA Kit
serum or plasma
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EIA-657C
HCV ELISA Kit
human serum or plasma
+Inquiry

EIA-664C
Hantavirus Dobrava / Hantaan IgG / IgM ELISA Kit
Human serum
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EIA-665C
Hantavirus (Puumala) IgG / IgM ELISA Kit
Human serum
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EIA-169C
Respiratory Syncytial Virus IgA ELISA Kit
serum and plasma
+Inquiry

Cat.#:	EIA-218C	Product Name:	Poliomyelitis; Poliomyelitis vieus; Polio
Sample:	serum and plasma	Species:
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Cat.#:	EIA-229C	Product Name:	Peanut ELISA Kit
Sample:	food	Species:
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Cat.#:	EIA-230C	Product Name:	Parainfluenza 1 / 2 / 3 IgM ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-231C	Product Name:	Parainfluenza 1 / 2 / 3 IgG ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-232C	Product Name:	Parainfluenza 1 / 2 / 3 IgA ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-241C	Product Name:	Mycoplasma pneumoniae IgM ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-242C	Product Name:	Mycoplasma pneumoniae IgG ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-243C	Product Name:	Mycoplasma pneumoniae IgA ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-244C	Product Name:	Mycobacterium tuberculosis IgM ELISA Kit, Sensitive
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-245C	Product Name:	Mycobacterium tuberculosis IgM ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-246C	Product Name:	Mycobacterium tuberculosis IgG ELISA Kit, Sensitive
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-247C	Product Name:	Mycobacterium tuberculosis IgG ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-248C	Product Name:	Mycobacterium tuberculosis IgA ELISA Kit, Sensitive
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-249C	Product Name:	Mycobacterium tuberculosis IgA ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-250C	Product Name:	Mycobacterium IgM ELISA Kit
Sample:	human serum	Species:
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Cat.#:	EIA-251C	Product Name:	Mycobacterium IgG ELISA Kit
Sample:	human serum	Species:
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Cat.#:	EIA-252C	Product Name:	Mycobacterium IgA ELISA Kit
Sample:	human serum	Species:
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Cat.#:	EIA-253C	Product Name:	MVV / CAEV ELISA Kit
Sample:	sheep serum and goat serum	Species:
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Cat.#:	EIA-254C	Product Name:	Mustard ELISA Kit
Sample:	food	Species:
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Cat.#:	EIA-255C	Product Name:	Mumps IgM ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-256C	Product Name:	Mumps IgG ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-257C	Product Name:	Mumps IgA ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-268C	Product Name:	Mouse Pyrene IgG ELISA Kit
Sample:	mouse serum.	Species:
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Cat.#:	EIA-270C	Product Name:	Mouse Phenanthrene IgG ELISA Kit
Sample:	mouse serum	Species:
+Inquiry

Cat.#:	EIA-275C	Product Name:	Mouse Naphthalene IgG ELISA Kit
Sample:	mouse serum.	Species:
+Inquiry

Cat.#:	EIA-566C	Product Name:	Human CMV IgG(CSF) ELISA kit
Sample:	serum and cerebrospinal fluid	Species:
+Inquiry

Cat.#:	EIA-567C	Product Name:	Human CMV IgG ELISA kit
Sample:	serum	Species:
+Inquiry

Cat.#:	EIA-568C	Product Name:	Human CMV IgG and IgG avidity ELISA kit
Sample:	serum	Species:
+Inquiry

Cat.#:	EIA-569C	Product Name:	Human CMV IgA ELISA kit
Sample:	serum and cerobrospinal fluid	Species:
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Cat.#:	EIA-320C	Product Name:	Mouse Fluorene IgG ELISA Kit
Sample:	mouse serum	Species:
+Inquiry

Cat.#:	EIA-321C	Product Name:	Mouse Fluoranthene IgG ELISA Kit
Sample:	mouse serum	Species:
+Inquiry

Cat.#:	EIA-329C	Product Name:	Mouse Chrysene IgG ELISA Kit
Sample:	mouse serum.	Species:
+Inquiry

Cat.#:	EIA-589C	Product Name:	Human BPDE-DNA ELISA Kit
Sample:	Human serum	Species:
+Inquiry

Cat.#:	EIA-334C	Product Name:	Mouse BPDE-DNA ELISA Kit
Sample:	mouse serum.	Species:
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Cat.#:	EIA-590C	Product Name:	Human Borrelia ELISA Kit
Sample:	PLASMA, SERUM OR CEREBROSPINAL FLUID	Species:
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Cat.#:	EIA-336C	Product Name:	Mouse Benzo(a)Pyrene ELISA Kit
Sample:	mouse serum.	Species:
+Inquiry

Cat.#:	EIA-338C	Product Name:	Mouse Anthracene IgG ELISA Kit
Sample:	mouse serum	Species:
+Inquiry

Cat.#:	EIA-595C	Product Name:	Human Benzo(a)Pyrene IgG ELISA Kit
Sample:	Human serum	Species:
+Inquiry

Cat.#:	EIA-596C	Product Name:	Human Benzo(a)Pyrene IgA ELISA Kit
Sample:	Human serum	Species:
+Inquiry

Cat.#:	EIA-342C	Product Name:	Mouse Acenaphthene IgG ELISA Kit
Sample:	mouse serum	Species:
+Inquiry

Cat.#:	EIA-598C	Product Name:	Human B. Burgdorferi ELISA Kit
Sample:	PLASMA, SERUM OR CEREBROSPINAL FLUID	Species:
+Inquiry

Cat.#:	EIA-347C	Product Name:	Measles IgM ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-348C	Product Name:	Measles IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-349C	Product Name:	Measles IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-350C	Product Name:	MC-LR ELISA Kit
Sample:	environmental samples	Species:
+Inquiry

Cat.#:	EIA-351C	Product Name:	MAP ELISA kit
Sample:	erum, plasma and milk samples from cattle, sheep and goat	Species:
+Inquiry

Cat.#:	EIA-353C	Product Name:	Lupin ELISA Kit
Sample:	food	Species:
+Inquiry

Cat.#:	EIA-355C	Product Name:	Influenza B IgM ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-356C	Product Name:	Influenza B IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-357C	Product Name:	Influenza B IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-359C	Product Name:	Influenza A IgM ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-360C	Product Name:	Influenza A IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-361C	Product Name:	Influenza A IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-623C	Product Name:	HSV-2 IgG ELISA Kit
Sample:	serum or plasma	Species:
+Inquiry

Cat.#:	EIA-624C	Product Name:	HSV1+2 IgG ELISA kit
Sample:	serum	Species:
+Inquiry

Cat.#:	EIA-625C	Product Name:	HSV-1 IgG ELISA Kit
Sample:	serum or plasma	Species:
+Inquiry

Cat.#:	EIA-370C	Product Name:	IgG4 Screen Nutritional 88 ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-371C	Product Name:	IgG4 Screen Nutritional 24 ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-372C	Product Name:	IgG4 Screen Nutritional 20 ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-373C	Product Name:	IgG Screen Nutritional ELISA Kit
Sample:	serum and plasma	Species:
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Cat.#:	EIA-374C	Product Name:	IgG Screen Nutritional 88 ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-638C	Product Name:	HiB IgG ELISA Kit
Sample:	Human Serum, Citrate-, EDTA- or Heparin Plasma	Species:
+Inquiry

Cat.#:	EIA-639C	Product Name:	HIA IgGAM ELISA Kit
Sample:	human plasma	Species:
+Inquiry

Cat.#:	EIA-384C	Product Name:	Human VCA EBV IgG ELISA kit
Sample:	serum	Species:
+Inquiry

Cat.#:	EIA-640C	Product Name:	HIA IgG ELISA Kit
Sample:	human plasma	Species:
+Inquiry

Cat.#:	EIA-385C	Product Name:	Human VCA EBV (CSF) IgG ELISA kit
Sample:	human serum and cerebrospinal fluid	Species:
+Inquiry

Cat.#:	EIA-642C	Product Name:	Herpes 2 IgM ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-643C	Product Name:	Herpes 2 IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-644C	Product Name:	Herpes 2 IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-645C	Product Name:	Herpes 1 / 2 IgM ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-646C	Product Name:	Herpes 1 / 2 IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-647C	Product Name:	Herpes 1 / 2 IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-648C	Product Name:	Herpes 1 IgM ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-649C	Product Name:	Herpes 1 IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-650C	Product Name:	Herpes 1 IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-653C	Product Name:	Helicobacter pylori IgM ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-654C	Product Name:	Helicobacter pylori IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-655C	Product Name:	Helicobacter pylori IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-656C	Product Name:	HCV ELISA Kit
Sample:	serum or plasma	Species:
+Inquiry

Cat.#:	EIA-657C	Product Name:	HCV ELISA Kit
Sample:	human serum or plasma	Species:
+Inquiry

Cat.#:	EIA-662C	Product Name:	Hazelnut ELISA Kit
Sample:	food	Species:
+Inquiry

Cat.#:	EIA-407C	Product Name:	Human thyroid peroxidase IgG ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-408C	Product Name:	Human thyroglobulin IgG ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-664C	Product Name:	Hantavirus Dobrava / Hantaan IgG / IgM ELISA Kit
Sample:	Human serum	Species:
+Inquiry

Cat.#:	EIA-665C	Product Name:	Hantavirus (Puumala) IgG / IgM ELISA Kit
Sample:	Human serum	Species:
+Inquiry

Cat.#:	EIA-672C	Product Name:	Gliadin ELISA Kit
Sample:	food	Species:
+Inquiry

Cat.#:	EIA-169C	Product Name:	Respiratory Syncytial Virus IgA ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-686C	Product Name:	Egg White ELISA Kit
Sample:	food	Species:
+Inquiry

Cat.#:	EIA-687C	Product Name:	Echinococcus IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-688C	Product Name:	EBV VCA IgM ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-689C	Product Name:	EBV VCA IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-690C	Product Name:	EBV VCA IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-691C	Product Name:	EBV EBNA-1 IgM ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-692C	Product Name:	EBV EBNA-1 IgG ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-693C	Product Name:	EBV EBNA-1 IgA ELISA kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-694C	Product Name:	EBV Early Antigen IgM ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-695C	Product Name:	EBV Early Antigen IgG ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-696C	Product Name:	EBV Early Antigen IgA ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-697C	Product Name:	dsDNA IgG ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry

Cat.#:	EIA-706C	Product Name:	Diphtheria Toxoid IgG ELISA Kit
Sample:	serum and plasma	Species:
+Inquiry