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Indirect ELISA Kit
The Indirect ELISA Kit is a widely used assay system designed for the detection and quantification of specific analytes such as proteins, antibodies, and other biomolecules within complex biological samples. This kit utilizes a two-step detection process that enhances sensitivity and flexibility compared to the direct ELISA method.
 
In an indirect ELISA, the process begins with the immobilization of the target antigen on the surface of the plate. A primary antibody, specific to the antigen, is then added to bind the target. After a washing step to remove unbound antibodies, a labeled secondary antibody is introduced. This secondary antibody is designed to recognize and bind to the host species of the primary antibody. The secondary antibody is typically conjugated to an enzyme, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Following a second wash step to remove unbound secondary antibodies, a substrate is added. The enzyme acts on the substrate to produce a color change, which can be measured to determine the presence and quantity of the target analyte.
 
Advantages of Indirect ELISA:
  • Higher Sensitivity: The use of a secondary antibody amplifies the signal, making indirect ELISA more sensitive than direct ELISA, allowing for the detection of lower concentrations of the target analyte.
  • Cost-Effective: Since the secondary antibodies are often commercially available and can be used with a variety of primary antibodies, this method is generally less expensive than direct ELISA.
  • Flexibility: The method is versatile, as it allows the use of many different primary antibodies, making it suitable for a wide range of applications.
 
Disadvantages of Indirect ELISA:
  • Risk of Cross-Reactivity: The primary disadvantage is the potential for cross-reactivity between the secondary antibody and other proteins in the sample, which can lead to nonspecific signals and reduced accuracy.
 
The Indirect ELISA Kit is particularly useful for applications where high sensitivity is required and flexibility in antibody selection is advantageous.

  • CAT. #
  • PRODUCT NAME
  • SAMPIE
  • PRICE
Cat.#: ADV-1616EK Product Name: Mouse anti-double stranded DNA antibody (IgG) ELISA Kit
Sample: Serum Species:
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Cat.#: ADV-1617EK Product Name: Mouse anti-cardiolipin antibody (IgG) ELISA Kit
Sample: Serum Species:
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Cat.#: ADV-1376EK Product Name: Human heparin (Hep) antibody (IgG) ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1377EK Product Name: Pig brucella antibody (IgG) ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1381EK Product Name: Human ganglioside D1b (GD1b) antibody (IgG) ELISA kit
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Cat.#: ADV-1384EK Product Name: Human enterovirus 71 virus (EV71) antibody (IgG) ELISA kit
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Cat.#: ADV-1394EK Product Name: Mouse anti respiratory syncytial virus (RSV) antibody (IgG)ELISA kit
Sample: Serum Species:
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Cat.#: ADV-1395EK Product Name: Human heparin (Hep) antibody (IgM) ELISA kit
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Cat.#: ADV-1401EK Product Name: Human RNA polymerase III (Pol III) antibody ELISA kit
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Cat.#: ADV-1412EK Product Name: Human rheumatoid factor (RF) antibody (IgM) ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1421EK Product Name: Human rheumatoid factor (RF) antibody (IgA) ELISA kit
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Cat.#: ADV-1442EK Product Name: Human Glutamate decarboxylase 1(GAD1) autoantibody ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1451EK Product Name: Human heparin (Hep) antibody (IgA) ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1453EK Product Name: Bovine anti-toxoplasmosis (TOXO) antibody (IgG) ELISA kit
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Cat.#: ADV-1455EK Product Name: Dog anti-toxoplasmosis (TOXO) antibody (IgG) ELISA kit
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Cat.#: ADV-1457EK Product Name: Human Zinc transporter 8 (SLC30A8) autoantibody ELISA kit
Sample: Serum, Plasma Species:
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Sample: Serum, Plasma Species:
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Cat.#: ADV-1458EK Product Name: Human alpha-enolase (ENO1) antibody (IgG) ELISA kit
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Cat.#: ADV-0434EK Product Name: Human ovalbumin specific IgE, OVA sIgE ELISA Kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-0435EK Product Name: Guinea pig ovalbumin specific IgE, OVA sIgE ELISA Kit
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Cat.#: ADV-1466EK Product Name: Human anti respiratory syncytial virus (RSV) antibody (IgG)ELISA kit
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Cat.#: ADV-1469EK Product Name: Human anti glial fibrillary acidic protein (GFAP) autoantibody ELISA kit
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Cat.#: ADV-1477EK Product Name: Human herpes simplex　virus Ⅰ+Ⅱ (HSVⅠ+Ⅱ) antibody (IgG) ELISA kit
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Cat.#: ADV-1486EK Product Name: Human high risk papillomavirus L1-capsids (HR-HPVL1) antibody (IgG) ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1487EK Product Name: Human papillomavirus type 18 L1-capsids(HPV18L1) antibody (IgG) ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1489EK Product Name: Human interleukin 8(IL8) autoantibody ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1514EK Product Name: Human anti-Helicobacter pylori (Hp) antibody (IgG) ELISA kit
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Cat.#: ADV-1532EK Product Name: Human parathyroid hormone 1-34(PTH 1-34)antibody ELISA kit
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Cat.#: ADV-0010EK Product Name: Human Tumor necrosis factor α (TNF-α) antibody ELISA kit
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Cat.#: ADV-1551EK Product Name: Dog rabies virus (RV) antibody (IgG) ELISA Kit
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Cat.#: ADV-0026EK Product Name: Pig Japanese Encephalitis Antibody(IgG)ELISA Kit
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Cat.#: ADV-0027EK Product Name: Pig Trichinella antibody(IgG) ELISA Kit
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Cat.#: ADV-1565EK Product Name: Rat helicobacter pylori (Hp) antibody (IgG) ELISA Kit
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Cat.#: ADV-0033EK Product Name: Human interferon γ(IFN-γ) antibody ELISA kit
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Cat.#: ADV-0034EK Product Name: Human voltage-gated potassium channel antibody (VGKC-ab) ELISA Kit
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Cat.#: ADV-0035EK Product Name: Human tuberculosis (TB) antibody (IgM) ELISA Kit
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Cat.#: ADV-0036EK Product Name: Human tuberculosis(TB) antibody ( IgG) ELISA Kit
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Cat.#: ADV-0037EK Product Name: Human Rabies Virus (RV) Antibody (IgG)ELISA Kit
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Cat.#: ADV-0039EK Product Name: Human Muscle Skeletal Receptor Tyrosine Kinase Antibody(MUSK Ab) ELISA Kit
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Cat.#: ADV-0040EK Product Name: Human melanocyte antibody, MC Ab ELISA Kit
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Cat.#: ADV-0041EK Product Name: Human hepatitis G virus (HGV) antibody (IgG)ELISA kit
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Cat.#: ADV-0042EK Product Name: Human Hepatitis E virus antibody(IgG)ELISA Kit
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Cat.#: ADV-1579EK Product Name: Rat keyhole limpet hemocyanin (KLH) antibody (IgG)ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-0043EK Product Name: Human anti-β1-adrenergic receptor(β1AR) autoantibody ELISA Kit
Sample: Serum Species:
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Cat.#: ADV-0044EK Product Name: Human anti-thrombopoietin receptor(C-MPL) autoantibody IgG ELISA kit
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Cat.#: ADV-0045EK Product Name: Human anti-streptolysin O antibody (IgG) ELISA Kit
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Cat.#: ADV-0046EK Product Name: Human anti-respiratory syncytial virus(RSV) antibody (IgM) ELISA Kit
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Cat.#: ADV-0047EK Product Name: Human Anti-nuclear Antibody, ANA ELISA Kit
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Cat.#: ADV-0048EK Product Name: Human anti-mutated citrullinated vimentin (MCV) antibody ELISA Kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-0049EK Product Name: Human anti-ganglioside (GM1)antibody(IgG)ELISA Kit
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Cat.#: ADV-0050EK Product Name: Human anti-filaggrin antibody, AFA ELISA Kit
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Cat.#: ADV-1590EK Product Name: Classical Swine Fever Virus Antibody(IgG) ELISA Kit
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Cat.#: ADV-1592EK Product Name: Rat islet cell antibody, ICA ELISA Kit
Sample: Serum Species:
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Cat.#: ADV-1594EK Product Name: Rat epidemic hemorrhagic fever antibody(IgG) ELISA kit
Sample: Serum Species:
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Cat.#: ADV-1595EK Product Name: Rat anti-toxoplasmosis (TOXO) antibody (IgG) ELISA kit
Sample: Serum Species:
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Cat.#: ADV-1597EK Product Name: Rat anti-streptolysin O antibody (IgG) ELISA Kit
Sample: Serum Species:
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Cat.#: ADV-1602EK Product Name: Mouse Chlamydia trachomatis(CT) antibody (IgG) Elisa kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1603EK Product Name: Mouse keyhole limpet hemocyanin (KLH) antibody (IgM) ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1604EK Product Name: Mouse keyhole limpet hemocyanin (KLH) antibody (IgG) ELISA kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1605EK Product Name: Mouse islet cell antibody, ICA ELISA Kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1606EK Product Name: Mouse insulin autoantibodies(IAA) ELISA Kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1608EK Product Name: Mouse Helicobacter pylori antibody(IgG)ELISA Kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1609EK Product Name: Mouse glutamic acid decarboxylase(GAD) autoantibody(IgM) ELISA Kit
Sample: Serum Species:
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Cat.#: ADV-1610EK Product Name: Mouse glutamic acid decarboxylase (GAD) autoantibody (IgG) ELISA Kit
Sample: Serum, Plasma Species:
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Cat.#: ADV-1611EK Product Name: Mouse anti-toxoplasmosis (TOXO) antibody (IgG) ELISA kit
Sample: Serum Species:
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Cat.#: EIA-713C Product Name: Cytomegaly IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-714C Product Name: Cow PAEP ELISA Kit
Sample: food Species:
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Cat.#: EIA-716C Product Name: CMV IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-718C Product Name: CMV IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-721C Product Name: CLA IgG ELISA Kit
Sample: sheep or goat sera Species:
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Cat.#: EIA-725C Product Name: Chlamydia Pneumoniae IgM ELISA Kit
Sample: human serum or plasma Species:
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Cat.#: EIA-726C Product Name: Chlamydia Pneumoniae IgG ELISA Kit
Sample: human serum or plasma Species:
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Cat.#: EIA-727C Product Name: Chlamydia Pneumoniae IgA ELISA Kit
Sample: human serum or plasma Species:
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Cat.#: EIA-737C Product Name: Casein ELISA Kit
Sample: food Species:
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Cat.#: EIA-738C Product Name: Candida albicans IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-739C Product Name: Candida albicans IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-740C Product Name: Candida albicans IgA ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-744C Product Name: Brucella IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-745C Product Name: Brucella IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-746C Product Name: Brucella IgA ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-747C Product Name: Breast CA15-3 ELISA Kit
Sample: human serum Species:
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Cat.#: EIA-765C Product Name: Borrelia burgdorferi IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-766C Product Name: Borrelia burgdorferi IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-767C Product Name: Bordetella pertussis IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-768C Product Name: Bordetella pertussis IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-769C Product Name: Bordetella pertussis IgA ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-772C Product Name: BHV1 gB ELISA kit
Sample: serum, plasma and milk samples from cattle Species:
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Cat.#: EIA-774C Product Name: Aspergillus Fumigatus IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-775C Product Name: Aspergillus Fumigatus IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-776C Product Name: Aspergillus Fumigatus IgA ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-791C Product Name: Almond ELISA Kit
Sample: food Species:
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Cat.#: EIA-795C Product Name: Adenovirus IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-796C Product Name: Adenovirus IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-797C Product Name: Adenovirus IgA ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-709C Product Name: Dengue IgM ELISA Kit
Sample: human serum Species:
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Cat.#: EIA-711C Product Name: Dengue IgG ELISA Kit
Sample: human serum Species:
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Cat.#: EIA-712C Product Name: Cytomegaly IgM ELISA Kit
Sample: serum and plasma Species:
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