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Indirect ELISA Kit
The Indirect ELISA Kit is a widely used assay system designed for the detection and quantification of specific analytes such as proteins, antibodies, and other biomolecules within complex biological samples. This kit utilizes a two-step detection process that enhances sensitivity and flexibility compared to the direct ELISA method.
 
In an indirect ELISA, the process begins with the immobilization of the target antigen on the surface of the plate. A primary antibody, specific to the antigen, is then added to bind the target. After a washing step to remove unbound antibodies, a labeled secondary antibody is introduced. This secondary antibody is designed to recognize and bind to the host species of the primary antibody. The secondary antibody is typically conjugated to an enzyme, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Following a second wash step to remove unbound secondary antibodies, a substrate is added. The enzyme acts on the substrate to produce a color change, which can be measured to determine the presence and quantity of the target analyte.
 
Advantages of Indirect ELISA:
  • Higher Sensitivity: The use of a secondary antibody amplifies the signal, making indirect ELISA more sensitive than direct ELISA, allowing for the detection of lower concentrations of the target analyte.
  • Cost-Effective: Since the secondary antibodies are often commercially available and can be used with a variety of primary antibodies, this method is generally less expensive than direct ELISA.
  • Flexibility: The method is versatile, as it allows the use of many different primary antibodies, making it suitable for a wide range of applications.
 
Disadvantages of Indirect ELISA:
  • Risk of Cross-Reactivity: The primary disadvantage is the potential for cross-reactivity between the secondary antibody and other proteins in the sample, which can lead to nonspecific signals and reduced accuracy.
 
The Indirect ELISA Kit is particularly useful for applications where high sensitivity is required and flexibility in antibody selection is advantageous.

  • CAT. #
  • PRODUCT NAME
  • SAMPIE
  • PRICE
  • EIA-112C
  • VZV IgG ELISA Kit
  • vitro diagnosis of VZV associated diseases, namely varicella and herpes zoster, including their neurological complications, i.e.
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Cat.#: EIA-101C Product Name: Yersinia IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-102C Product Name: Yersinia IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-103C Product Name: Yersinia IgA ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-104C Product Name: Wheat Gliadin IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-105C Product Name: Wheat Gliadin IgG ELISA Kit
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Cat.#: EIA-106C Product Name: Wheat Gliadin IgA ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-107C Product Name: Walnut ELISA Kit
Sample: food Species:
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Cat.#: EIA-108C Product Name: VZV IgM ELISA Kit
Sample: serum Species:
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Cat.#: EIA-109C Product Name: VZV IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-110C Product Name: VZV IgG ELISA Kit
Sample: serum Species:
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Cat.#: EIA-111C Product Name: VZV IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-112C Product Name: VZV IgG ELISA Kit
Sample: vitro diagnosis of VZV associated diseases, namely varicella and herpes zoster, including their neurological complications, i.e. Species:
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Cat.#: EIA-113C Product Name: VZV IgG and IgG adidity ELISA Kit
Sample: Human Serum Species:
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Cat.#: EIA-115C Product Name: VCA EBV IgM ELISA Kit
Sample: serum Species:
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Cat.#: EIA-116C Product Name: VCA EBV IgG ELISA Kit
Sample: serum Species:
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Cat.#: EIA-117C Product Name: Varizella zoster IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-118C Product Name: Varizella zoster IgG ELISA Kit
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Cat.#: EIA-119C Product Name: Varizella zoster IgA ELISA Kit
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Cat.#: EIA-121C Product Name: Trypanosoma cruzi IgG ELISA Kit
Sample: human serum Species:
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Cat.#: EIA-122C Product Name: Trypanosoma cruzi ELISA Kit
Sample: serum or plasma Species:
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Cat.#: EIA-123C Product Name: Tropomyosin ELISA Kit
Sample: food Species:
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Cat.#: EIA-128C Product Name: Toxoplasma IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-129C Product Name: Toxoplasma IgG ELISA Kit
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Cat.#: EIA-137C Product Name: Tetanus Toxoid IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-138C Product Name: Tetanospasmin ELISA Kit
Sample: Human serum and human plasma Species:
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Cat.#: EIA-140C Product Name: TBEV (FSME) IgM ELISA kit
Sample: serum and plasma Species:
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Cat.#: EIA-141C Product Name: TBEV (FSME) IgG ELISA kit
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Cat.#: EIA-142C Product Name: TBE IgM ELISA Kit
Sample: Human Serum, Plasma and Cerebrospinal Fluid (CSF) Species:
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Cat.#: EIA-143C Product Name: TBE IgG ELISA Kit
Sample: Human Serum, Plasma and Cerebrospinal Fluid (CSF) Species:
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Cat.#: EIA-144C Product Name: T. gondii IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-146C Product Name: T. gondii IgG ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-147C Product Name: T. Cruzi IgG ELISA Kit
Sample: human serum Species:
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Cat.#: EIA-157C Product Name: Soy ELISA Kit
Sample: food Species:
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Cat.#: EIA-158C Product Name: Sesame ELISA Kit
Sample: food Species:
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Cat.#: EIA-162C Product Name: Rubella IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-163C Product Name: Rubella IgG ELISA Kit
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Cat.#: EIA-164C Product Name: RUBELLA IgG ELISA Kit
Sample: human serum Species:
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Cat.#: EIA-165C Product Name: Rheumatoid Factor IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-166C Product Name: Rheumatoid Factor IgA ELISA Kit
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Cat.#: EIA-167C Product Name: Respiratory Syncytial Virus IgM ELISA Kit
Sample: serum and plasma Species:
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Cat.#: EIA-168C Product Name: Respiratory Syncytial Virus IgG ELISA Kit
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