Cat#:PA-1306F;Product Name:Mouse Anti-Human MLH1 Antibody;Synonym:FCC2; COCA2; HNPCC; hMLH1; HNPCC2; MGC5172; DNA mismatch repair protein Mlh; OTTHUMP00000161361; OTTHUMP00000208782; OTTHUMP00000210160; OTTHUMP00000210161; OTTHUMP00000210162; mutL protein homolog 1;Background:Mismatch repair gene hMLH1 is a ubiquitous gene encoding a mismatch repair protein (MMR) called MutLprotein homolog 1 (MLH1) which is utilized by normal proliferating cells to repair point mutations that may occurduring DNA replication. MLH1 forms a heterodimer with the mismatch repair protein MutL protein homolog 2(PMS2) and the MLH1-PMS2 heterodimeric complex is recruited to the mismatch DNA sequence by a MutSMMR heterodimeric complex consisting of the MSH2-MSH6 heterodimer, which binds directly to the basemismatch repair error. The MLH1-PMS2 complex then initiates downstream repair functions including theexcision of the mismatched DNA strand and repair through the recruitment of nucleases, polymerases, and otherassorted proteins. The mismatched nucleotides, or microsatellite instable (MSI) sequence, targeted by theheterodimer are consequently repaired as the result of normal dimerization of the two proteins in an ATPdependentprocess.MLH1 deficiency is often the result of germline mutations in MMR deficient individuals. Absence of MLH1 hasalso been reported in 10-15% of sporadic colorectal carcinomas. Individuals lacking the MLH1 protein arepredisposed for hereditary non-polyposis colorectal cancer (HNPCC). HNPCC is an autosomal dominant disorderassociated with a high risk for developing colorectal cancer. Additionally, HNPCC increases the risk forextracolonic cancers including carcinoma of the endometrium, ovary, renal pelvis, small bowel, stomach andureter. HNPCC is believed to account for approximately 2- 5% of all colorectal cancers and about 50% ofHNPCC mutations occurring in the MutL homologue 1 gene.Antibodies to MLH1 are useful for identifying mismatch repair deficiencies in tumors of the gastrointestinal tractincluding HNPCC and associated extracolonic cancers by immunohistochemistry (IHC). MLH1 deficiency asdetermined by IHC has been reported in 80.3% of MSI-High colon carcinomas and 12.4% of endometrial carcionomas associated with HNPCC.;Description:Mouse Anti-Human MLH1 Monoclonal Antibody;Host Species:Mouse;Species Reactivity:Human;Clone#:ES16;Isotype:IgG1;Application:IHC;Storage:Store antibody products at 2-8°C. For long term storage, aliquot and freeze at -20°C. Avoid repeated freeze/thaw cycles;Usage:For Lab Research Use Only;