Cat#:RP-6075H;Product Name:Recombinant Human TGFA Protein;Synonym:Transforming Growth Factor Alpha, Protransforming Growth Factor Alpha, TGF-Alpha, TGFA.;Description:TGFA Protein (40-89) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6kDa. The TGFA is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:The ED50, as measured in a proliferation assay using mouse Balb/3T3 cells, is less than 0.4ng/ml.;Formulation:Lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;