Cat#:RP-5623H;Product Name:Recombinant Human SCF Protein, Sf9;Synonym:Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.;Description:Stem Cell Factor Protein produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is fused to a C-terminal His-tag (6xHis) and purified by proprietary chromatographic techniques.;Source:Sf9, Insect Cells.;Purity:Greater than 98.0% as determined by analysis by SDS-PAGE.;Bioactivity:The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml.;Formulation:The protein is supplied in 1xPBS, pH 7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Stem Cell Factor in 10mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;