Cat#:RP-4293H;Product Name:Recombinant Human Leptin Protein;Synonym:OB Protein, Obesity Protein, OBS, Obesity factor.;Description:Leptin Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa. The Leptin is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.;Purity:Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.;Formulation:The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.;Storage:Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C.;