Cat#:RP-4292H;Product Name:Recombinant Human LEFTY1 Protein;Synonym:Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B, LEFTY1, LEFTB, LEFTYB.;Description:LEFTY1 Protein produced in HEK cells is a glycosylated monomer, having a total molecular weight of 38kDa. The LEFTY1 is purified by proprietary chromatographic techniques.;Source:HEK.;Purity:Greater than 90% as obsereved by SDS-PAGE.;Bioactivity:The specific activity was determined by dose dependent ability to inhibit BMP-4 (6.5 ng/mL) induction of alkaline phosphatase production in the MC-3T3-E1 cell line (Mouse chondrogenic cell line) and is typically 10-40ng/ml.;Formulation:The LEFTY1 was lyophilized from 1mg/ml in 1xPBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized LEFTY1 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized?LEFTY1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LEFTY1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B, LEFTY1, LEFTB, LEFTYB.
Description:
LEFTY1 Protein produced in HEK cells is a glycosylated monomer, having a total molecular weight of 38kDa. The LEFTY1 is purified by proprietary chromatographic techniques.
Source:
HEK.
Purity:
Greater than 90% as obsereved by SDS-PAGE.
Bioactivity:
The specific activity was determined by dose dependent ability to inhibit BMP-4 (6.5 ng/mL) induction of alkaline phosphatase production in the MC-3T3-E1 cell line (Mouse chondrogenic cell line) and is typically 10-40ng/ml.
Formulation:
The LEFTY1 was lyophilized from 1mg/ml in 1xPBS.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized LEFTY1 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized?LEFTY1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LEFTY1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.