Cat#:RP-4064H;Product Name:Recombinant Human IL3 Protein, HEK;Synonym:MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.;Description:Interleukin-3 Protein produced in HEK cells is a glycosylated monomer, having a molecular weight range of 17-45kDa due to glycosylation. The IL-3 is purified by proprietary chromatographic techniques.;Source:HEK.;Purity:Greater than 90% as obsereved by SDS-PAGE.;Bioactivity:The activity was determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.5-2ng/ml corresponding to a specific activity of 500,000-2,000,000units/mg.;Formulation:The IL3 was lyophilized from 1mg/ml in 1xPBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized IL-3 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized?IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Interleukin-3 Protein produced in HEK cells is a glycosylated monomer, having a molecular weight range of 17-45kDa due to glycosylation. The IL-3 is purified by proprietary chromatographic techniques.
Source:
HEK.
Purity:
Greater than 90% as obsereved by SDS-PAGE.
Bioactivity:
The activity was determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.5-2ng/ml corresponding to a specific activity of 500,000-2,000,000units/mg.
Formulation:
The IL3 was lyophilized from 1mg/ml in 1xPBS.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized IL-3 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized?IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.