Cat#:RP-4105H;Product Name:Recombinant Human IL1A Protein, HEK;Synonym:Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.;Description:Interleukin-1 alpha Protein produced in HEK cells is a glycosylated monomer, having a molecular weight of 18kDa due to glycosylation. The IL1A is purified by proprietary chromatographic techniques.;Source:HEK.;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:Assay # 1: The biological activity was measured by its binding ability in a functional ELISA, the ED50 is typically 0.5-5 µg/ml. Assay # 2: The biological activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells, ;Formulation:The IL-1A protein was lyophilized from a 0.2µm filtered solution of PBS pH 7.4 with 10% trehalose as protectant.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized IL1A in deionized water to a stock solution of 0.5mg/ml.;Storage:Lyophilized IL-1A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;