Cat#:RP-4015H;Product Name:Recombinant Human IL10 Protein, HEK;Synonym:B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.;Description:Interleukin-10 Protein produced in HEK cells is a glycosylated non-disulfide bonded homodimer, having a total molecular weight of 17kDa. The IL10 is purified by proprietary chromatographic techniques.;Source:HEK.;Purity:Greater than 95% as obsereved by SDS-PAGE.;Bioactivity:The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of 200 pg/ml IL-4, and is typically 0.3-1.5ng/ml.;Formulation:The IL10 was lyophilized from 1mg/ml in 10mM Tris-HCl, pH 7.4 and 150mM NaCl.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized IL-10 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized?IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Interleukin-10 Protein produced in HEK cells is a glycosylated non-disulfide bonded homodimer, having a total molecular weight of 17kDa. The IL10 is purified by proprietary chromatographic techniques.
Source:
HEK.
Purity:
Greater than 95% as obsereved by SDS-PAGE.
Bioactivity:
The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of 200 pg/ml IL-4, and is typically 0.3-1.5ng/ml.
Formulation:
The IL10 was lyophilized from 1mg/ml in 10mM Tris-HCl, pH 7.4 and 150mM NaCl.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized IL-10 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized?IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.