Cat#:RP-3994H;Product Name:Recombinant Human IGFBP1 Protein;Synonym:IBP-1, IGF-Binding Protein 1,hIGFBP-1, AFBP, PP12, IGF-BP25,IGFBP-1.;Description:Recombinant human IGFBP1 Protein (26-259 a.a.) expressed in NS0 is a single, glycosylated, polypeptide chain containing 234 amino acids and having a molecular mass of 25kDa. The recombinant human IGFBP1 protein is purified by our unique chromatographic techniques.;Source:Mouse myeloma cell line, NS0.;AA Sequence:APWQCAPCSA EKLALCPPVS ASCSEVTRSA GCGCCPMCAL PLGAACGVAT ARCARGLSCR ALPGEQQPLH ALTRGQGACV QESDASAPHA AEAGSPESPE STEITEEELL DNFHLMAPSE EDHSILWDAI STYDGSKALH VTNIKKWKEP CRIELYRVVE SLAKAQETSG EEISKFYLPN CNKNGFYHSR QCETSMDGEA GLCWCVYPWN GKRIPGSPEI RGDPNCQIYF NV;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:The ED50 of recombinant human IGFBP1 protein, as determined by the inhibition of rHuIGF-I-induced proliferation of human MCF-7 cells, is less than 4µg/ml.;Formulation:Recombinant human IGFBP1 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS.;Stability:Recombinant human IGFBP1 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized IGFBP1 protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human IGFBP1 protein although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution recombinant human IGFBP1 protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.;References:Ekstrand J, Ehrenborg E, Stern I, et al. (1990). "The gene for insulin-like growth factor-binding protein-1 is localized to human chromosomal region 7p14-p12". Genomics. 6 (3): 413–8.;