Cat#:RP-3993H;Product Name:Recombinant Human IGF2 Protein;Synonym:Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.;Description:Recombinant human Insulin-Like Growth Factor-II / IGF2 Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7505 Dalton. Recombinant human IGF2 protein is purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.;Purity:Greater than 97.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50 of recombinant human IGF2 protein, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a Specific Activity of 1,176,470IU/mg.;Formulation:The human IGF2 protein was lyophilized with no additives.;Stability:Recombinant Human IGF2 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized human IGF2 protein in sterile 18MΩ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human IGF2 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human IGF2 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.;References:Stromal-derived IGF2 promotes colon cancer progression via paracrine and autocrine mechanisms. Unger C, et al. Oncogene, 2017 Sep 21. PMID 28534511;