Cat#:RP-3977H;Product Name:Recombinant Human IFN a 2b Protein, Yeast;Synonym:Interferon alpha 2b, IFNA, INFA2, IFN-? 2b, MGC125764, MGC125765.;Description:Interferon-alpha 2b Protein produced in yeast is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of approximately 19 kDa. The Interferon-alpha 2b gene was obtained from human leukocytes. The IFN-a 2b is purified by proprietary chromatographic techniques.;Source:Saccharomyces cerevisiae.;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln.;Purity:Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The specific activity as determined in a viral resistance assay was found to be no less than 300,000,000IU/mg.;Formulation:Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH-7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized glycosilated IFN alpha 2b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized glycosilated IFN-a 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Interferon-alpha 2b Protein produced in yeast is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of approximately 19 kDa. The Interferon-alpha 2b gene was obtained from human leukocytes. The IFN-a 2b is purified by proprietary chromatographic techniques.
Source:
Saccharomyces cerevisiae.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln.
Purity:
Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bioactivity:
The specific activity as determined in a viral resistance assay was found to be no less than 300,000,000IU/mg.
Formulation:
Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH-7.4.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized glycosilated IFN alpha 2b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized glycosilated IFN-a 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.