Cat#:RP-4084H;Product Name:Recombinant Human IL7 Protein, Yeast;Synonym:Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.;Description:Interleukin-7 Protein produced in yeast is a single, glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17.4 kDa. The IL-7 is purified by proprietary chromatographic techniques.;Source:Saccharomyces cerevisiae.;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu.;Purity:Greater than 98.0% as determined by SDS-PAGE.;Bioactivity:The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine pre-B cell line 2E8 is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 106 units/mg.;Formulation:Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;