Cat#:RP-3215H;Product Name:Recombinant Human FGF2 Protein, Thermostable;Synonym:Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.;Description:FGF-Basic Thermostable is a stabilized form of FGF-2 growth factor which enables a novel method to produce FGF2-dependent cell cultures more efficiently, having less media changes. FGF-Basic Thermostable can maintain its biological activity even after five days at 37°C. The increase in the stability of FGF2 in cell-culture enables a more homogenous, undifferentiated stem cell culture, while saving scientists crucial time and money, as frequent supplementation of FGF-basic and the everyday medium change is not necessary. Thermostable FGF-2 is a hyperstable protein. The Thermal stability of the protein is increased by 15°C compared to the wild-type FGF-2. Thermostable FGF-2 is more than 5-times prolonged half-life in human cell culture incubated at 37°C. The FGF-2 Thermostable protein is engineered with fully retained biological function and has no harmful stabilizing additives. Thermostable FGF2 Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids including a 20 aa His tag and thrombin cleavage recognition site at N-terminus. The total calculated molecular mass is 19.4kDa.;Source:E.coli;Purity:Purity is Greater than 95% as determined by SDS-PAGE.;Formulation:FGF2 was filtered and lyophilized from a solution containing 20mM potassium phosphate buffer and 750mM sodium chloride, pH 7.5.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5-1mg/ml and let the lyophilized pellet dissolve completely.;Storage:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.;