Cat#:RP-3211H;Product Name:Recombinant Human FGF2 Protein (147 a.a.);Synonym:Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.;Description:Fibroblast Growth Factor-2 Protein (FGF-2) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.5kDa. The FGF2 is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS.;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:The ED50, Calculated by the dose- dependent proliferation of mouse BALB/c 3T3 cells is 0.018-0.027ng/ml corresponding to a specific activity of 5.6x107 units/mg.;Formulation:The bFGF was lyophilized from a sterile filtered solution containing 10mM sodium phosphate, 75mM sodium chloride, pH 7.5.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized FGF-B in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized basic-FGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGFb should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;