Cat#:RP-2156H;Product Name:Recombinant Human b NGF Protein, HEK;Synonym:Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.;Description:Beta-NGF Protein produced in HEK cells is a non-glycosylated non-disulfide linked homodimer, having a total molecular weight of 13kDa. The b-NGF is purified by proprietary chromatographic techniques.;Source:HEK.;Purity:Greater than 95% as obsereved by SDS-PAGE.;Bioactivity:The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line), the ED50 is <4ng/ml.;Formulation:The b-NGF was lyophilized from 1mg/ml in 1xPBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized b-NGF in sterile 1xPBS containing 0.1% endotoxin-free recombinant HSA.;Storage:Lyophilized b-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution b-NGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Beta-NGF Protein produced in HEK cells is a non-glycosylated non-disulfide linked homodimer, having a total molecular weight of 13kDa. The b-NGF is purified by proprietary chromatographic techniques.
Source:
HEK.
Purity:
Greater than 95% as obsereved by SDS-PAGE.
Bioactivity:
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line), the ED50 is <4ng/ml.
Formulation:
The b-NGF was lyophilized from 1mg/ml in 1xPBS.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized b-NGF in sterile 1xPBS containing 0.1% endotoxin-free recombinant HSA.
Storage:
Lyophilized b-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution b-NGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.