Cat#:RP-2271HAT;Product Name:Recombinant Human PKM2 Protein;Synonym:pyruvate kinase muscle isoform M2;Background:PKM, the full name is pyruvate kinase M1/2. Gene ID: 5315. This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011];Description:The human recombinant PKM2, produced in E.coli, was fused with His tag at N Terminus. This recombinant protein was purified using our unique purification.;Source:E.coli;AA Sequence:1-531aa;Molecular Characterization:60.1 kDa;Purity:Greater than 95% by SDS-PAGE;Endotoxin:< 1.0 EU per ug of protein as determined by LAL method.;Bioactivity:Specific activity: > 25,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.;Formulation:Liquid Solution;Stability:Recombinant Human PKM2 Protein was stable up to 1 year from date of receipt at -70℃;Host Species:Human;Concentration:1mg/mL;Storage Buffer:Recombinant Human PKM2 protein was supplied in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol;Storage:Recombinant Human PKM2 Protein can be stored at +4℃for 1-2 weeks. It is recommended that the recombinant protein be aliquoted for optimal storage at -20 or -70℃. Please avoid repeated freeze-thaw cycles.;Usage:For Lab Research Use Only;References:LincRNA-p21 suppresses development of human prostate cancer through inhibition of PKM2. Wang X, et al. Cell Prolif, 2017 Dec. PMID 28994148;