Cat#: | RP-6194H |
Product Name: | Recombinant Human TPI1 Protein, Active |
Synonym: | TPI, TIM, Triosephosphate Isomerase 1. |
Description: | TPI1 produced in E.coli is a single, non-glycosylated polypeptide chain containing 269 amino acids (1-249a.a.) and having a molecular mass of 28.8kDa. TPI1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Source: | E.coli |
AA Sequence: | MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GS |
Purity: | Greater than 95% as determined by SDS-PAGE. |
Bioactivity: | Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C. |
Formulation: | The TPI1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Storage: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles. |