Cat#:EIA-510C;Product Name:Human IgM ELISA Kit;Size:96T;Sample:serum, plasma, cerebrospinal fluid, milk, and urine;Intended use:RUO;Synonym:Constant region of heavy chain of IgM; Hepatitis B virus receptor binding protein; Ig mu chain C region; IGHM; Immunoglobulin mu chain; Imunoglobulin heavy chain; VH; IgM; Immunoglobulin M;Background:The immunoglobulins (Ig) are the second most abundant proteins in human blood after albumin. These proteins are used by the immune system to bind to and neutralize foreign molecules entering the bloodstream. Of the five immunoglobulin isotypes (IgA, IgD, IgE, IgG, and IgM), immunoglobulin M (IgM) is the only isotype that is present in an immature B-cell. Upon maturation and activation, the cells begin to produce the other immunoglobulin isotypes. As a pentamer of identical subunits, IgM is the largest protein in the circulatory system and constitutes about 10% of all immunoglobulins in serum.The most important role of IgM undoubtedly lies in its ability to function early in the immune responses against blood-borne pathogens. As might be expected from its pentameric structure, IgM is very efficient in agglutinating particulate antigens and is particularly effective at complement activation. Since IgM antibodies appear early in the course of an infection, the levels of IgM in the bloodstream is useful in the diagnosis of recent infection. In addition, since IgM antibodies do not pass across the human placenta, its presence in a neonate's serum likely indicates intrauterine infection. Therefore, an accurate quantitation of IgM in plasma or serum is important for medical diagnostics, as well as for monitoring the efficacy of treatment regimens.;Species Reactivity:Human;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;