Cat#:EIA-512C;Product Name:Human IgG ELISA Kit;Size:96T;Sample:serum, plasma, cerebrospinal fluid, milk, and urine;Intended use:RUO;Synonym:IgG; Ig gamma 1 chain C region; IGHG1; Immunoglobin heavy constant gamma 1; Immunoglobulin G;Background:The immunoglobulins (Ig) are the second most abundant proteins in human blood after albumin. These proteins are used by the immune system to bind and neutralize foreign molecules entering the bloodstream. Of the five immunoglobulin isotypes (IgA, IgD, IgE, IgG, and IgM), immunoglobulin G (IgG) is the most abundant and constitutes about 75% of the total serum immunoglobulins. The IgG concentration in normal human serum ranges from 6 mg/ml to 18 mg/ml.The relative abundance of the different isotypes is generally constant in mature adults. Changes in the relative abundance of the different isotypes usually indicate immunological disorders or disease states. For example, congenital and acquired immunodeficiency results in significantly reduced levels of IgG, while hepatitis, cirrhosis, and most autoimmune diseases increase the IgG concentrations. Therefore, an accurate quantitation of IgG in plasma or serum is important for medical diagnostics, as well as for monitoring the efficacy of treatment regimens.;Species Reactivity:Human;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;