Cat#:EIA-531C;Product Name:Human Free T4 ELISA Kit;Size:96T;Sample:human serum;Intended use:in vitro diagnosis;Synonym:1,3,5-Triaza-1,3,5-Trinitrocyclohexane; 1,3,5-triaza-1,3,5-trinitrocyclohexane[qr]; 1,3,5-Triazacyclohexane 1,3,5-trinitro-; cx84a; Cyclonite; Cyclotrimethylenenitramine; Esaidro-1,3,5-trinitro-1,3,5-triazina; Geksogen; Heksogen; heksogen(polish); Hexogeen; Hexogen (explosive); Hexogen 5W; hexogen(explosive); khp281; PBX(AF) 108; pbx(af)108; PBXW 108(E); pbxw108(e); pe4; RDX; HEXOGEN; Free Thyroxine;Background:L-Thyroxine (T4) or 3,5,3’,5’-tetraiodothyronine is the most commonly measured thyroid hormone for the diagnosis of thyroid function. T4 has its primary influence on protein synthesis and oxygen consumption in virtually all tissues but it is also important for growth, development, and sexual maturation. T4 is synthesised by the thyroid gland and is secreted into the bloodstream. Here the T4 becomes bound to serum proteins for transport to the cells. The major transport protein is Thyroxine Binding Globulin (TBG) which normally accounts for 80% of the bound T4. Other thyroid hormone binding proteins are Thyroxine Binding Prealbumin and Albumin. Most of the serum T4 is bound to these transport proteins leaving only about 0.03% free to exert its effect on cells. It is the free T4 (fT4) that represents the metabolically active fraction; for this reason the measurement of fT4 concentration is considered to be an indicator of patient thyroid status. Primary hypothyroidism results in underproduction of T4 by the thyroid gland and consequently an abnormally low circulating fT4 concentration is in the blood. Primary hyperthyroidism leads to excessive thyroid production on T4 and resulting elevated fT4 concentration. Total serum T4 concentrations are dependent on the level of circulating TBG as well as the patient’s thyroid status. The concentration of TBG can be affected by certain drugs, steroid hormones, pregnancy, and by various nonthyroid illnesses. In an earlier generation of thyroid function tests, the effect of variable TBG concentration was dealt with by calculating a Free Thyroxine Index (FTI). This FTI is the product of Total T4 concentration and Thyroid Uptake (TU), which assesses the number of available binding sites on the TBG. This approach requires carrying out two separate assay determinations (total T4 and TU), but does provide a better indicator of thyroid status than total T4 alone. fT4 tests are designed to directly reflect the equilibrium existing in serum between T4 and TGB-bound T4. These methods, including the fT4 tests, can generally reflect thyroid status in a single assay.;Species Reactivity:Human;Application:quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Competitive ELISA;
L-Thyroxine (T4) or 3,5,3’,5’-tetraiodothyronine is the most commonly measured thyroid hormone for the diagnosis of thyroid function. T4 has its primary influence on protein synthesis and oxygen consumption in virtually all tissues but it is also important for growth, development, and sexual maturation. T4 is synthesised by the thyroid gland and is secreted into the bloodstream. Here the T4 becomes bound to serum proteins for transport to the cells. The major transport protein is Thyroxine Binding Globulin (TBG) which normally accounts for 80% of the bound T4. Other thyroid hormone binding proteins are Thyroxine Binding Prealbumin and Albumin. Most of the serum T4 is bound to these transport proteins leaving only about 0.03% free to exert its effect on cells. It is the free T4 (fT4) that represents the metabolically active fraction; for this reason the measurement of fT4 concentration is considered to be an indicator of patient thyroid status. Primary hypothyroidism results in underproduction of T4 by the thyroid gland and consequently an abnormally low circulating fT4 concentration is in the blood. Primary hyperthyroidism leads to excessive thyroid production on T4 and resulting elevated fT4 concentration. Total serum T4 concentrations are dependent on the level of circulating TBG as well as the patient’s thyroid status. The concentration of TBG can be affected by certain drugs, steroid hormones, pregnancy, and by various nonthyroid illnesses. In an earlier generation of thyroid function tests, the effect of variable TBG concentration was dealt with by calculating a Free Thyroxine Index (FTI). This FTI is the product of Total T4 concentration and Thyroid Uptake (TU), which assesses the number of available binding sites on the TBG. This approach requires carrying out two separate assay determinations (total T4 and TU), but does provide a better indicator of thyroid status than total T4 alone. fT4 tests are designed to directly reflect the equilibrium existing in serum between T4 and TGB-bound T4. These methods, including the fT4 tests, can generally reflect thyroid status in a single assay.
Species Reactivity:
Human
Application:
quantitative
Usage:
For Lab Research Use Only
Detection Principle:
Competitive ELISA
Storage:
Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.
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