Cat#:EIA-532C;Product Name:Human Free T3 ELISA Kit;Size:96T;Sample:human serum;Intended use:in vitro diagnosis;Synonym:triiodothyronine,reverse; TRIIODOTHYRONINE; REVERSE T3; T3; LIOTHYRONIN; L-3,3',5'-TRIIODOTHYRONINE; (3,5,3')-TRIIODO-L-THYRONINE; Reverse T3; 3,3',5'-triiodo-L-thyronine free acid; Isoliothyronine; 3,3’,5-Triiodo-L-thyronine-13C6; 3,3’,5-Triiodothyronine-13C6; Liothyronine-13C6; L-T3-13C6; O-(4-Hydroxy-3-iodophenyl)-3,5-diiodo-L-tyrosine-13C6; T3 (Hormone)-13C6; Tresitope-13C6; Triiodothyronine-13C6; Free Triiodothyronine;Background:Triiodothyronine (3, 5, 3’-triiodo-I-thyronine, T3) and Thyroxine (T4) are two active hormones found in the bloodstream. Approximately 20% of circulating T3 is derived from direct synthesis and secretion by the thyroid gland, while 80% is produced by the deiodination of T4 in peripheral tissues. T3 is transported through the peripheral bloodstream primarily bound to serum proteins, specifically Thyroxine Binding Globulin (TBG), Thyroid Binding Prealbumin (TBPA) and Albumin. Only about 0.3% of the total serum T3 is unbound and free to diffuse into tissues to exert its biological effects. T3 has its primary influence on the rate of oxygen consumption and heat production in almost all tissues. The hormone also plays a critical role in the growth, development and sexual maturation of growing mammals. Total serum T3 is one parameter used in the differentiation and clinical diagnosis of thyroid disease, in particular Hyperthyroidism. In most hyperthyroid patients both T3 and T4 are elevated. Approximately 5-10% of all cases of hyperthyroidism, however, have elevated T3 concentrations accompaniedby normal T4 concentrations which is known as T3-thyrotoxicosis. Such clinical conditions make it vital to establish that T3 levels are normal before excluding the diagnosis of hyperthyroidism. Serum T3 levels are also an excellent indicator of the ability of the thyroid to respond to stimulation and suppression test. T3 Enzyme-Immunoassay provides a rapid, sensitive method for measuring T3 in unextracted serum using T3 antibody and enzyme labelled T3 Conjugate.;Species Reactivity:Human;Application:quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Competitive ELISA;