Cat#:EIA-627C;Product Name:Horse IL-2 ELISA Kit;Size:96 T;Sample:cell culture supernatants;Intended use:RUO;Synonym:TCGF; interleukin 2; interleukin-2; T-cell growthfactor; lymphokine; IL-2;Background:IL-2 is a member of a family of cytokines; which also includes IL-4; IL-7; IL-9; IL-15 and IL-21. IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25); IL-2 receptor beta (CD122) and a common gamma chain (γc); which is shared by all members of this family of cytokines. Binding of IL-2 activates the Ras/MAPK; JAK/Stat and PI 3-kinase/Akt signaling modules. IL-2 is normally produced by the body during an immune response.1-2 When environmental substances (molecules or microbes) gain access to the body; these substances are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of IL-2; and the expression of IL-2 receptors IL-2R. The IL-2/IL-2R interaction then stimulates the growth; differentiation and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes.3-5 As such; IL-2 is necessary for the development of T cell immunologic memory; one of the unique characteristics of the immune system; which depends upon the expansion of the number and function of antigen-selected T cell clones. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (Tregs). 6-8 After exiting from the thymus; T-Regs function to prevent other T cells from recognizing and reacting against "self antigens"; which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2.9 Thus; IL-2 is required to discriminate between self and nonself; another one of the unique characteristics of the immune system. IL-2 has been found to be similar to IL-15 in terms of function.10 Both cytokines are able to facilitate production of immunoglobulins made by B cells and induce the differentiation and proliferation of natural killer cells.11-12 The primary differences between IL-2 and IL-15 are found in adaptive immune responses. For example; IL-2 participates in maintenance of T-Regs and reduces selfreactive T cells. On the other hand; IL-15 is necessary for maintaining highly specific T cell responses by supporting survival of CD8 memory T cells.;Species Reactivity:Horse;Application:Quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;