Cat#:ADV-0201EK;Product Name:Rat Tri-iodothyronine, T3 ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Cell Culture Supernates, Tissue Homogenates;Description:This Rat Tri-iodothyronine (T3) ELISA Kit was designed for the quantitative measurement of Rat Tri-iodothyronine (T3) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-8 ng/mL and the sensitivity is 0.5 ng/mL.;Species Reactivity:Rat;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate --The 96-well plate has been pre-coated with an anti-human Ts antibody. Standard(10 x concentrate) (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-conjugated T3 (1 x 6 ml) HRP-avidin (1 x 6 ml) --Catalyze the TMB, eliciting a chromogenic reaction. Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances. Substrate A (1x 7 ml) --Mix with Substrate B. Substrate B (1x 7 ml) --Mix with Substrate A. Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:0.5 ng/mL-8 ng/mL;Sensitivity:0.5 ng/mL;
This Rat Tri-iodothyronine (T3) ELISA Kit was designed for the quantitative measurement of Rat Tri-iodothyronine (T3) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-8 ng/mL and the sensitivity is 0.5 ng/mL.
Species Reactivity:
Rat
Usage:
For Research Use Only
Detection Principle:
Competitive ELISA
Contents of Kit:
A 96-well Assay plate --The 96-well plate has been pre-coated with an anti-human Ts antibody. Standard(10 x concentrate) (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-conjugated T3 (1 x 6 ml) HRP-avidin (1 x 6 ml) --Catalyze the TMB, eliciting a chromogenic reaction. Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances. Substrate A (1x 7 ml) --Mix with Substrate B. Substrate B (1x 7 ml) --Mix with Substrate A. Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual