Cat#:ADV-0202EK;Product Name:Mouse Tri-iodothyronine, T3 ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Tissue Homogenates;Description:The mouse Tri-iodothyronine (T3) ELISA kit is used to detect and quantify the concentrations of T3 in serum, plasma, and tissue homogenates. This kit exclusively recognizes mouse T3. It adopts the competitive inhibition enzyme immunoassay technique in which Biotin-conjugated T3 and T3 in samples or standard compete for binding to the pre-coated T3 antibody. And HRP catalyzes TMB to elicit a chromogenic reaction. The intensity of the color is inversely proportional to the amount of T3 in the sample. This ELISA kit has been confirmed to have high sensitivity, strong specificity, premium precision, and lot-to-lot consistency. See the instructions for more details. T3 is a hormone produced by the thyroid gland and plays an important role in the body's control of metabolism. Mariane de Oliveira etc. demonstrated that T3 induced a decrease in lipid accumulation through the oxidation of fatty acids in adipocytes and improved oxidative stress and DNA damage. T3 elevated the production of adipokines, adiponectin, and leptin with a concomitant deduction in PPARy and FNDC5 levels.;Species Reactivity:Mouse;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:An assay plate --- The 96-well plate has been pre-coated with an anti-mouse T3 antibody. Standard (6 x 1 ml) --- Reconstitute one of the standards with sample diluent at dilution series and draw the standard curve. Biotin-conjugated T3 (1 x 6 ml) HRP-avidin (1 x 6 ml)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use. Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away the unbound solution and non-specific substances. Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal. Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal. Stop Solution (1 x 7 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow. Four Adhesive Strip (For 96 wells) --- Cover the microtiter plate when incubation. An instruction manual;Detection Range:0.5 ng/mL-8 ng/mL;Sensitivity:0.5 ng/mL;