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Antibody / Antibody Pairs
 In a sandwich ELISA, which is a highly sensitive and specific format used for detecting antigens, the antibody pair plays a crucial role. This pair consists of two types of antibodies: a capture antibody and a detection antibody. Each antibody is selected for its ability to bind to a different epitope on the target antigen, ensuring the specificity of the assay.
 
Capture Antibody: This antibody is coated onto the surface of the ELISA plate wells. Its primary function is to capture and immobilize the antigen from the sample being tested. The selection of a high-affinity capture antibody is vital for the efficiency of antigen capture and the overall sensitivity of the assay.
Detection Antibody: After the antigen is captured and immobilized by the capture antibody, the detection antibody is added to the assay. This antibody is designed to bind to a different epitope on the target antigen than the one recognized by the capture antibody. The detection antibody can be directly labeled with an enzyme or a tag (in direct detection methods) or can be recognized by a secondary antibody that is enzyme-labeled (in indirect detection methods).
The combination of these two antibodies, specifically targeting distinct sites on the antigen, forms the basis of the "sandwich" in the sandwich ELISA format. This method provides high sensitivity due to the amplification of the signal through the enzymatic reaction and high specificity due to the dual recognition of the antigen.
 
For capture and detection antibodies to be effectively used in a sandwich ELISA experiment, they must meet certain criteria:
  • Specificity: Both antibodies must be highly specific to different epitopes on the target antigen to avoid cross-reactivity with other proteins in the sample, which could lead to false positives.
  • Affinity: High affinity is crucial, especially for the capture antibody, to ensure stable and strong binding of the antigen from the sample. The detection antibody also needs a high affinity to ensure that it can effectively bind to the antigen that's been captured on the plate.
  • Minimal Cross-Reactivity: Both antibodies should have minimal cross-reactivity to other components in the sample to reduce background noise and improve assay specificity.
  • Stability: Antibodies should maintain their stability and binding capabilities under the assay conditions, including the buffering systems, washing steps, and incubation times and temperatures.
 
The future prospects for antibody pairs, particularly in the context of diagnostics and therapeutic applications, are closely tied to advancements in antibody engineering and biotechnological innovations. With the ongoing development of bispecific antibodies technology, the applications of antibody pairs are expanding into more sophisticated diagnostic tools and therapeutic strategies.

Antibody / Antibody Pairs
Species
  • CAT. #
  • PRODUCT NAME
  • SOURCE
  • PRICE
Cat.#: ADC-57265A Product Name: abscisic acid Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57264A Product Name: Ractopamine Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57263A Product Name: estradiol Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57262A Product Name: Ofloxacin Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57261A Product Name: Sulfadimidine Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57260A Product Name: Sulfametoxydiazine Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57259A Product Name: Chenodeoxycholic acid Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57258A Product Name: Corticosterone Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57257A Product Name: Dehydroepiandrosterone(DHEA) Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57256A Product Name: Gentamicin Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57255A Product Name: Clenbuterol Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57254A Product Name: Kanamycin Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57253A Product Name: FITC Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57252A Product Name: Folic acid Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57251A Product Name: The metabolite of furazolidone Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57250A Product Name: Enrofloxacin Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57249A Product Name: Testosterone Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57248A Product Name: Cortisol Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57247A Product Name: Cholic acid Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57246A Product Name: The metabolite of furaltadone Monoclonal Antibody
Source (Host): Species: Rabbit
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Cat.#: ADC-57245A Product Name: The metabolite of nitrofurazone Monoclonal Antibody
Source (Host): Species: Mouse
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Cat.#: ADC-57244A Product Name: Neomycin Monoclonal Antibody
Source (Host): Species: Mouse
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