Cat#:PA-230F;Product Name:Mouse Anti-Human TOP2A Antibody;Synonym:TOP2A; topoisomerase (DNA) II alpha 170kDa; TOP2; TP2A; DNA topoisomerase 2-alpha; DNA gyrase; OTTHUMP00000207108; DNA topoisomerase II, 170 Kd; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase II, alpha isozyme;Background:DNA topoisomerases are ubiquitous enzymes that control DNA topology by cleaving and rejoining DNA strands. There are two major types of DNA topoisomerases, topo I and II, which are functionally distinct. Topo I cuts one strand of DNA, whereas topo II cuts both strands of DNA at one time in an ATP-dependent reaction. Studies on topo ll from vertebrates, including humans, have revealed the existence of two isoforms. Topo IIα with a molecular mass of 170 kDa, and topo IIβ with a molecular mass of 180 kDa. The two isoforms normally exist as homodimers, but a small proportion of αβ heterodimers has been detected in cultured cells. The two isoforms are highly similar, but genetically distinct, and display different expression patterns. Topo IIα is expressed in proliferating cells in late S phase, and with a peak in G2-M phases , where it is believed to be the primary mediator of chromosome condensation. Topo IIα is not expressed in the G0 phase, representing resting and non-proliferating cells. In contrast, topo IIβ is constantly expressed once the cells enter the cell cycle and is still present in the G0 phase.;Description:Mouse Anti-Human TOP2A Monoclonal Antibody;Host Species:Mouse;Species Reactivity:Human;Clone#:Ki-T2;Isotype:IgG2a, kappa;Application:IHC;Storage:Store antibody products at 2-8°C. For long term storage, aliquot and freeze at -20°C. Avoid repeated freeze/thaw cycles;Usage:For Lab Research Use Only;