Cat#:PA-1340F;Product Name:Mouse Anti-Human MCM3 Antibody;Synonym:MCM3; minichromosome maintenance complex component 3; HCC5; P1.h; RLFB; MGC1157; P1-MCM3; DNA replication licensing factor MCM3; p102; RLF subunit beta; hRlf beta subunit; DNA replication factor MCM3; OTTHUMP00000016600; cervical cancer proto-oncogene 5; minichromosome maintenance deficient 3; replication licensing factor, beta subunit; MCM3 minichromosome maintenance deficient 3; EC 3.6.4.12; DNA polymerase alpha holoenzyme-associated protein P1; Cervical cancer proto oncogene 5; DNA polymerase alpha holoenzyme associated P1; DNA polymerase alpha holoenzyme associated protein P1; HCC 5; Human cervical cancer proto oncogene 5; MCM 3; Minichromosome maintenance protein 3; P1 h; P1 MCM3; P1 Protein; P102 protein; Replication licensing factor beta subunit; RLF beta subunit;Background:The exact duplication of a genome once per cell division is required of every proliferating cell. In eukaryotic cells this is achieved by temporally separating the assembly of a pre-replication complex (pre-RC) from initiation of DNA synthesis at replication origins. A key component of the pre-RC is the hexameric MCM complex composed of the highly conserved proteins MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. This MCM complex is also a DNA helicase. To ensure that initiation of DNA synthesis occurs only once per cell division, the recruitment of the MCM complex is temporally separated from its activation. In G1 phase, the MCM complex is recruited to the pre-RC in an inactive globular conformation. In S phase, the MCM complex becomes phosphorylated, which leads to a conformational change that converts the inactive MCM complex into an enzymatically active helicase that becomes topologically linked to DNA and disassociates from the pre-RC. This again leads to the melting of dsDNA and recruitment of proteins involved in DNA synthesis. Like Ki-67 antigen, MCM3 protein is expressed in proliferating cells. However, MCM3 protein disappears more slowly after initiation of differentiation than the Ki-67 antigen, and antibodies against MCM3 protein label more cells than antibodies against Ki-67 antigen. This observation, correlated with the fact that MCM3 protein is not expressed when a marker of terminal differentiation, such as p27, is expressed, indicates that antibodies against MCM3 protein label cells in the early stages of cell cycle exit, i.e. cells that have not terminally differentiated. Antibodies against MCM3 protein in combination with a panel of other antibodies may identify dysplastic cells that remain in cycle owing to deregulation of normal controls over cell proliferation as a step in their progression from normal to neoplastic cells.;Description:Mouse Anti-Human MCM3 Monoclonal Antibody;Host Species:Mouse;Species Reactivity:Human;Clone#:112;Isotype:IgG1, kappa;Application:IHC;Storage:Store antibody products at 2-8°C. For long term storage, aliquot and freeze at -20°C. Avoid repeated freeze/thaw cycles;Usage:For Lab Research Use Only;