Cat#:PA-2758F;Product Name:Mouse Anti-Gentamicin Antibody;Synonym:Gentamicin; GENTAMICIN; GENTAMYCIN; GENTAMYCINE; GentamysinsolutionforBiochemistry; Gentacycol; Gentavet; GENTAMICINUM; Refobacin tm; Uromycine; Gentamicin (base and/or unspecified salts); Lyramycin;Background:This monoclonal antibody was generated from Balb/c mice immunized with gentamicin-KLH conjugate. Its antigen-binding ability was shown by its specific recognition of gentamicin that has been conjugated in BSA by ELISA and immune-gold based lateral flow assay, respectively; the recognition is inhibited in the presence of free gentamicin in the test samples. No significant cross-reaction to other unrelated antibiotics tested (including Penicillin G, Ampicilline, Tetracycline, kanamycine and neomycin) was detected by competitive ELISA. This antibody can be used as a detecting reagent in assays designed to quantitatively or semi-quantitatively measure gentamicin, such as competitive ELISA or immunochromatography-based lateral flow.In addition, for ELISA in combination with the gentamicin-BSA conjugate as coating antigen and HRP-conjugated goat-anti-mouse IgG as secondary antibody, this antibody at as lower as 5 ng/ml (i.e., 1: 200, 000 dilutions of 1 mg/ml) was shown to still give decent OD readings after addition of relevant substrates. However, the optimal working dilutions for each specific application should be determined by the user empirically.;Description:Mouse Anti-Gentamicin Monoclonal Antibody;Host Species:Mouse;Species Reactivity:N/A;Clone#:G4-0;Isotype:IgG2a;Application:ELISA, LF;Storage:Store antibody products at 2-8°C. For long term storage, aliquot and freeze at -20°C. Avoid repeated freeze/thaw cycles;Usage:For Lab Research Use Only;