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Recombinant CAS9 S. Pyogenes Protein Online Inquiry

Cat#:RP-2374H
Product Name:Recombinant CAS9 S. Pyogenes Protein
Synonym: CRISPR-associated endonuclease Cas9/Csn1, csn1, SpyCas9, cas9,
Gene Introduction: Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to memorizeand later interrogate and cleave foreign DNA,such as invading bacteriophage DNA or plasmid DNA.
Description: Recombinant Cas9-NLS expressed in E.coli, comprises the entire Cas9 protein sequence (1368aa) joined to a proprietary nuclear localization sequence (NLS) and a 6xHis tag at the C-terminus, having a total of 1414aa.
Source: E.coli
Molecular Characterization: The protein has an apparent molecular weight of 163kDa.
Purity: Greater than 90% as determined by SDS-PAGE.
Bioactivity: ENZYMATIC ACTIVITY: 97% of PCR product digestion after 30 minutes at 37°C. ENDONUCLEASE ACTIVITY: The incubation of 20pmol of CAS9 protein with 1µg pUC19 supercoiled vector at 37°C for 4 hours leads to formation of less than 10% open circular pUC19 form as determined by gel electrophoresis. NON-SPECIFIC DNASE ACTIVITY: The incubation of 20pmol of CAS9 protein with 1µg of lambda DNA at 37°C for 4 hours is free of detectable DNA degradation, as visualized on agarose gel.
Formulation: The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.
Stability: Recombinant Cas9 Proteins are stable for up to 1 year from date of receipt at -70℃
Storage: Store CAS9 S. Pyogenes Protein at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
References: Heler, R; Samai, P; Modell, J. W.; Weiner, C; Goldberg, G. W.; Bikard, D; Marraffini, L. A. (2015). "Cas9 specifies functional viral targets during CRISPR–Cas adaptation". Nature. 519 (7542): 199–202.

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