Cat#:RP-2294R;Product Name:Recombinant Rat CTF1 Protein;Synonym:Cardiotrophin-1, CT-1, Ctf1.;Description:Cardiotrophin-1 Rat Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 203 amino acids and having a molecular mass of 21.4kDa. The CTF1 Rat is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA.;Purity:>95.0% as determined by SDS-PAGE and HPLC analyses.;Bioactivity:The ED50 as determined by the dose-dependent proliferation of TF-1 cells was found to be < 0.5ng/ml, corresponding to a specific activity of > 2.0×106 units/mg.;Formulation:Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Cardiotrophin-1 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF1 Rat should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;