Cat#: | RP-2364R |
Product Name: | Recombinant Rat M CSF Protein |
Synonym: | Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csf1, Csfm. |
Description: | Macrophage Colony Stimulating Factor Rat Recombinant produced in E.coli is a non-glycosylated homodimer, containing 2 x 155 amino acids and having a total molecular mass of 36.2 kDa. MCSF is purified by proprietary chromatographic techniques. |
Source: | E.coli |
AA Sequence: | MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP. |
Purity: | >95.0% as determined by SDS-PAGE. |
Bioactivity: | The activity is determined by dose-dependent induction of M-NFS-60 cell proliferation and is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5x105 units/mg. |
Formulation: | Lyophilized from a sterile filtered aqueous solution containing 10mM Na3PO4, pH 7.5. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized MCSF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage: | Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |