Cat#: | RP-2359R |
Product Name: | Recombinant Rat Leptin Protein |
Synonym: | OB Protein, Obesity Protein, OBS, Obesity factor. |
Description: | Leptin Rat Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16 kDa. The Leptin is purified by proprietary chromatographic techniques. |
Source: | E.coli |
AA Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-His. |
Purity: | >95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. |
Bioactivity: | Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage: | Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |