Cat#: | RP-2336R |
Product Name: | Recombinant Rat IL15 Protein |
Synonym: | IL-15, MGC9721. |
Description: | Interleukin-15 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 13533 Dalton. The IL-15 is purified by proprietary chromatographic techniques. |
Source: | E.coli |
AA Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asn-Trp-Ile-Asp. |
Purity: | >98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Bioactivity: | The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 10 ng/ml, corresponding to a Specific Activity of 100,000IU/mg. |
Formulation: | The protein was lyophilized from 10mM Tris, pH-8.5. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage: | Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL15 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |