Cat#: | RP-2302R |
Product Name: | Recombinant Rat ENA78 / CXCL5 Protein |
Synonym: | C-X-C motif chemokine 5, Small-inducible cytokine B5, Cytokine LIX, Cxcl5, Scyb5, LIX, GCP-2, Scyb6, ENA-78, AMCF-II. |
Gene Introduction: | CXCL5 (C-X-C Motif Chemokine Ligand 5) is a Protein Coding gene. Diseases associated with CXCL5 include Pediatric Ulcerative Colitis and Acute Cervicitis. Among its related pathways are PEDF Induced Signaling and Akt Signaling. |
Description: | Epithelial Neutrophil-Activating Protein 78 Rat Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 93 amino acids and having a molecular mass of 10.0kDa. The CXCL5 is purified by proprietary chromatographic techniques. |
Source: | E.coli |
AA Sequence: | APFSAMVATE LRCVCLTLAP RINPKMIANL EVIPAGPHCP KVEVIAKLKN QKDNVCLDPQ APLIKKVIQK ILGSENKKTK RNALALVRSA STQ. |
Purity: | >97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Bioactivity: | Determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 10.0-100.0 ng/ml. |
Formulation: | Lyophilized from a 0.2µm filtered concentrated (1.0mg/ml) solution in 1×PBS, pH 7.4. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized ENA-78 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Host Species: | Rat |
Storage: | Lyophilized ENA-78 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
References: | Solution structure of CXCL5--a novel chemokine and adipokine implicated in inflammation and obesity. (PMID: 24695525) Sepuru KM … Rajarathnam K PloS one 2014 |