Cat#:RP-3536AT;Product Name:Recombinant Rat Tnf Protein;Synonym:Tumor necrosis factor;Description:Recombinant rat Tnf, fused with His tag at N Terminus, was produced in E.coli. This recombinant protein was purified using our unique chromatography techniques.;Source:E.coli;AA Sequence:80-235aa;Molecular Characterization:19.9kDa;Purity:Greater than 90% by SDS-PAGE;Endotoxin:< 1.0 EU per 1ug of protein as determined by LAL method.;Bioactivity:Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 0.2 ng/ml.;Formulation:Liquid Solution;Stability:Recombinant Rat Tnf Protein was stable up to 1 year from date of receipt at -70℃;Concentration:1mg/mL;Storage Buffer:Recombinant Rat Tnf protein was supplied in Phosphate Buffer Saline (pH7.4) containing 10% glycerol, 1mM DTT;Storage:Recombinant Rat Tnf Protein can be stored at +4℃for 1-2 weeks. It is recommended that the recombinant protein be aliquoted for optimal storage at -20 or -70℃. Please avoid repeated freeze-thaw cycles.;Usage:For Lab Research Use Only;