Cat#:RP-1968M;Product Name:Recombinant Mouse sRANKL (158-316) Protein;Synonym:Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RAN;Description:sRANKL Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain (158-316 a.a) having a molecular mass of 19kDa. The sRANKL is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:Lys158-Asp316, with an N-terminal Met, Accession # Q3TWY5.;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:The ED50, as measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells, is less than 2ng/ml.;Formulation:sRANKL protein was lyophilized from a 0.2µm filtered solution in NaH2PO4, NaCl and EDTA.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized sRANKL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;