Cat#:RP-1963M;Product Name:Recombinant Mouse SHH Protein;Synonym:SHH, HHG-1, HHG1, Sonic hedgehog protein.;Description:Sonic Hedgehog Recombinant Mouse produced in E.coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8 kDa. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. The Sonic HedgeHog is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG.;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:Determined by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.48-0.72 µg/ml.;Formulation:Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM Na2PO4 pH-7.5.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Sonic Hedgehog in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Mouse Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;