Cat#:RP-1683M;Product Name:Recombinant Mouse C5a Protein;Synonym:Complement C5, Hemolytic complement, C5, Hc, He, C5a.;Description:C5a Mouse Recombinant produced in E.coli is a single,?non-glycosylated, Polypeptide chain containing?77 amino acids and having a molecular mass of 9kDa. The Mouse C5a is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Leu-His-Leu-Leu.;Purity:Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.;Formulation:Mouse C5a was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.5 and 350mM NaCl.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;